It is necessary to follow the course of Prof.ssa Bucci Cecilia

Transfection of mammalian cells. Use of GFP to monitor transfection efficiency. Transfection of GFP-tagged proteins to establish their intracellular localization in mammalian cells. Immunofluorescence analysis. Differentiation of cultured mammalian cells. Staining of Lipid Droplets in 

The course aims to provide technical skills to work professionally with roles of responsibility in the areas of medical biotechnology which make use of eukaryotic cells (wt or genetically modified).

Pratical laboratory classes (1 cfu = 10 hours)

Assessment is not expected

-BIOLOGIA CELLULARE E GENETICA: Parte Prima - Biologia Cellulare a cura di Fantoni, Bozzaro, Del Sal, Ferrari - Casa Editrice PICCIN.

-MOLECULAR BIOLOGY OF THE CELL: Alberts et al., VII edition, Garland Science.